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CADs for a Troubleshoot-Free Flow Cell for FSCV Calibrations

Updated: May 4, 2023

Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) is a method traditionally used for real-time quantification of neurotransmitters in biological systems. Reliable calibration of CFMs is essential for converting FSCV signals to analyte concentrations and generally employs flow injection analysis (FIA) performed with flow cells fabricated in-house. Such FSCV FIA cells often require significant and ongoing troubleshooting with pulsing, leaking, flow inconsistencies and dead volume being major causes of common challenges. In this work, we address these issues by creating a robust, plug-and-play FSCV flow cell. This novel design permits reproducible, high-precision, and stable flow injection profiles using low-cost materials to improve FSCV calibration. The ready-to-print computer-aided designs and hardware list are provided.


The flow cell design and part list can be found below, and for further information, please visit https://pubs.acs.org/doi/10.1021/acsmeasuresciau.2c00059


Part

Quantity (per flow cell)

Supplier

Stainless steel standoff, Male-Female, 40 mm, 46 mm

6

Farnell

M3 dome nuts

3

Accu

M3 x 8mm thumb screws

3

Accu

M3 x 6mm countersunk screw

3

Accu

¼-28 hex nut

1

Zoro


FlowCell
.zip
Download ZIP • 2.07MB

Authors: Melissa Hexter, Joseph van Batenburg-Sherwood, and Parastoo Hashemi

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